Alloxaninduced rats,antidiabetes, diabetes, ipomoea batatas, phytochemical analysis. The aim of this study was to evaluate the antihyperglycemic effects of gbe on streptozotocin stz induced diabetes in rats. Alloxan toxin causes diabetes suzy cohen will give you free. The mandibular incisors of onemonthold rats born to these mothers were analyzed. Whether after 10 to 60 min incubation of pieces of pancreas in the presence of 214calloxan or 60 min to 24 h after intravenous injection of 214calloxan to. The hypoglycemic effect of the kelp on diabetes mellitus. Diabetes was induced in albino rats by administration of a single dose of alloxan monohydrate 5% 125 mgkg, i. Kelp powder was added to the general forage for the rats.
In diabetes mellitus the body cannot secrete enough insulin or it cannot use its. Alloxan is a urea derivative which causes selective necrosis of the. The uptake of 214calloxan by the pancreatic gland was investigated in control and streptozotocininduced diabetic stz rats, using both in vitro and in vivo techniques. Alloxan monohydrate is used to induce diabetes mellitus in albino rats in the dose of 120mgkg i. Rats were fast overnight before injection with alloxan. The hemimandibles were removed and submitted to radiographical and histopathological procedures. Antihyperglycemic and antihyperlipidemic effects of. Alloxan diabetes definition of alloxan diabetes by medical. So the total hemoglobin level is lowered in alloxan induced diabetic rats. In research it is used for induction of type 1 diabetes. Nigerian honey ameliorates hyperglycemia and dyslipidemia in.
Insulin and alpha amylase levels in alloxaninduced diabetic. A single dose study was studied in the normal rats for 12hrs. Another group of fasted rats was administered normal saline without alloxan. Diabetes was induced in wistar rats by single intraperitoneal injection of alloxan monohydrate in sterile normal saline to overnight fasted animals at a dose of 120 mgkg b. Rats treated with alloxan 120mgkg, for 2 consecutive days, showed an increase in the. The clinical course of the diabetes mortality, hyperglycemia, weight loss, polydipsia, hyperphagia, polyuria, glycosuria and diabetic indices was recorded for six weeks before the animals were sacrificed for volumetric quantitation of the pancreatic islets. Of immediate interest was the question of whether permanent diabetes could be produced in other animals. The mechanism of alloxan and streptozotocin action in b. Blood glucose levels were measured daily 3 days prior and 7 days after alloxan administration. Passiflora edulis, antidiabetic, hyperglycemia, alloxan mono hydrate introduction nsulin is the principal hormone secreted by. Histopathological study of the liver of alloxan induced. Research article alloxan induced diabetes mellitus in wistar. Histopathological assessment of the kidney of alloxan. Pdf antidiabetic and hypolipidemic effect of asparagus.
Diabetes mellitus, allium sativum, histopathology, alloxan, kidney and weaning rats cite this article as. To determine the effect of parquetina nigrescens extract on fasting blood glucose in alloxan induced diabetic rats. Antihyperlipidemic and antiperoxidative effect of diasulin, a. Increased levels of plasma glucose in alloxan induced diabetic rats were lowered by the administration of c.
Apr 06, 2018 optimization of alloxan dose is essential to induce stable diabetes for prolonged period alloxan dose for experimental induction of diabetes in rats has to be optimized. To elucidate the pharmacological basis of the antidiabetic efficacy of this plant drug, the effect of r. Diabetes mellitus, adansonia digitata, anterior pituitary, wistar rats. The blood glucose levels, complete blood count, erythrocyte indices and osmotic fragility, body and organ. Histopathological study of the liver of alloxan induced diabetic rats and macerated allium sativum garlic ameliorative effect.
The animals were divided into 4 groups each of 10 rats. Chromatographic analysis identified the presence of the flavonoids rutin, isoquercetin, and kaempferitrin. The fasting blood glucose level was determined after 72 hours of alloxan injection. Alloxan, in the presence of intracellular thiols, generates reactive oxygen species ros in a cyclic reaction with its reduction product, dialuric acid. The mechanisms of alloxan and streptozotocininduced diabetes. Lower doses can result in autoreversion to normal state without medical help, while higher doses cause toxicity and loss of experimental animals. Allium sativum, alloxan, diabetes mellitus, histopathology, liver and weaning rats. Enoobong bassey, samson oyebadejo and asuquo ikanna. Groups consisted of pregnant rats supplemented isdr or not nisdr with insulin and controls, in which sterile saline solution was administered instead of alloxan or insuli n. Fabaceae leaves and flowers on alloxaninduced diabetes in rats.
Alloxan induced diabetes has been observed to cause a massive reduction of the beta cells of the islets of pancreas leading to hyperglycemia. The diabetic rats which showed blood glucose levels in the range of 19. Alloxaninduced diabetes, a common model for evaluating the. The hypolipidemic effect of leaf essential oil of citrus sinensis in alloxaninduced diabetic rats was evaluated. Antidiabetic effect of costus speciosus rhizome extract in.
Samson oyebadejo, enoobong bassey, ajayi oyewunmi, victor archibong and ekaete usoro. Acute eemn treatment reduced hyperglycemia, improved oral glucose tolerance, and minimized dyslipidemia and oxidative stress leading to a reduction in atherogenic index in alloxaninduced diabetic rats. Sixty healthy male rats were used to establish dm models by injecting alloxan intraperitoneally. Introduction diabetes mellitus diabetes is a group of metabolic diseases in which a person has high blood sugar, either because the body does not produce enough insulin, or because cells do not respond to the insulin that is. Antidiabetic effect of ipomoea batatas in normal and alloxan. Diabetes was induced in wistar rats n 25 by intravenous administration of alloxan 42 mgkg and were analyzed at 1, 3, 6, 9 and 12 months after diabetes induction. Alloxan is most prominent chemical compound used in diabetogenic research. Photomicrographs of rat pancreas stained by haematoxylin and eosin of a untreated and b alloxan induced diabetic rats and effects of. The mechanism of alloxan and streptozotocin action in b cells. The researchers stated, these hydroxyl radicals are ultimately responsible for the death of the beta cells, which have a particularly. Antioxidant and hypoglycemic activity of strawberry fruit. Brazilian morus nigra attenuated hyperglycemia, dyslipidemia. Studies suggests that alloxan does not cause diabetes in humans.
The effect of aqueous leaf extract of adansonia digitata. Thereafter, fbg was recorded at days 3, 7, 14, 21, 28, and 35 to determine fluctuations in fbg or autoreversal of diabetes. Diabetes follows the alloxaninduced destruction of the pancreatic islet beta cell and, in the appropriate dose, it is selective to islet. Thirty rats were used divided into 2 groups, normal control. In rabbits, a very high mortality of about 80% was seen with rapid i. Autoxidation of dialuric acid generates superoxide radicals. Preventive effects of flavonoids on alloxaninduced diabetes. To investigate the hypoglycemic and hypolipidemic activities of hydroalcoholic extract of echinochloa frumentacea link grains in alloxan induced diabetic rat. Alloxaninduced diabetes triggers the development of. This study was aimed to determine the glycemic and antioxidant effects of vco in alloxan induced diabetic rats. Antidiabetic and hypolipidemic activity of punica granatum.
Alloxan induced diabetes in mice protocol diabetestalk. Alloxan is a cyclicurea derivative, which is a highly effective, diabetogenic agent used widely as a tool to induce experimental diabetes. Composed from 10 rats and diabetic group composed from 20 rats i. The beta cell toxic action of alloxan is initiated by free radicals formed in this redox reaction. Acute eemn treatment reduced hyperglycemia, improved oral glucose tolerance, and minimized dyslipidemia and oxidative stress leading to a reduction in atherogenic index in. It has been successfully induced in a variety of animal species.
Turmeric curcuma longa is one of the common constituents of our daily food. Alloxaninduced diabetes is a form of insulindependent diabetes mellitus that occurs as a result of alloxan administration or injection to animals. We report herein the findings of this research work. Induction and treatment of diabetes diabetes was induced by a single injection of alloxan 60 mgkg b.
Forty albino rats 150200 g were randomly selected into 4 groups of 10 rats each, representing normal control, diabetic control, diabetic treated with 14. The effect of orally administered aqueous extracts 400 mgkg body weight of clitoria ternatea leaves and flowers on serum glucose, glycosylated hemoglobin, and insulin were examined in control and extracttreated diabetic rats. Pubmed used the cytotoxic compound alloxan monohydrate to induce diabetes in swiss albino mice. This article was published in diabetologia and it was entitled the mechanisms of alloxan and streptozotocininduced diabetes.
Alloxan induced a dosedependent mortality in 25%, 33. Alloxaninduced insulin release is, however, of short duration and is followed by complete suppression of the islet response to glucose, even when high concentrations 16. Diabetes was induced in male wistar rats by the administration of stz 60 mgkg b. Preventive effects of flavonoids on alloxaninduced. Hypoglycemic effects and the use of kelp in diabetes mellitus dm model rats induced by alloxan were investigated. Enamel hypoplasia in a litter of rats with alloxaninduced. Antidiabetic activity of ethanolic extract of tubers of. Diabetes mellitus was induced in overnight fasted male wistar rats 180220 g via intraperitoneal administration of 150 mgkg body weight bw of alloxan dissolved in normal saline. Diabetes was induced in rats with equal molar dosages of either streptozotocin or alloxan. Dec 18, 2007 alloxan and streptozotocin are toxic glucose analogues that preferentially accumulate in pancreatic beta cells via the glut2 glucose transporter. The rats were randomly grouped into four as follows. The animals were deprived of food for 48 hours, and then weighed and anaesthesized with chloroform in a glass dome. Diabetes was induced by a single intraperitoneal injection with alloxan 100 mg kg body weight lenzen, 2008. Comparison of streptozotocin and alloxaninduced diabetes in.
Alloxan and streptozotocin are toxic glucose analogues that preferentially accumulate in pancreatic beta cells via the glut2 glucose transporter. Show full abstract investigate the protective and curative effects of camel milk and ginkgo biloba extract against alloxan induced diabetes in rats. Histopathological assessment of the kidney of alloxan induced. Determination of the efficacy of the active fraction in alloxan diabetic rats the alloxan diabetic rats were divided into three groups of six each. The present study wa s undertaken to evaluate the antidiabetic effects of ethanolic extract of rhizomes of curcuma longa in alloxan induced diabetic rats and compared with of pioglitazone, which is the standard antidiabetic agent.
Diabetes follows the alloxan induced destruction of the pancreatic islet beta cell and, in the appropriate dose, it is selective to islet beta cell, producing minimal effects on other structures. Antidiabetic and antioxidant effects of virgin coconut oil. Antidiabetic, antihypercholestermic and antioxidative effect. Alloxan definition of alloxan by medical dictionary. Alloxaninduced diabetes, a common model for evaluating. In the presence of intracellular thiols, especially glutathione, alloxan generates reactive oxygen species ros in a cyclic redox reaction with its reduction product, dialuric acid. The plant, parquetina nigrescens is used in folklore medicine to treat diabetes mellitus and its complications in several parts of west africa. Antihyperglycemic effect of ginkgo biloba extract in. Alloxan has been administered in single or multiple doses, through different routes intraperitoneal, intravenous and subcutaneous. Also, this convenient method of producing a permanent experimental pancreatic diabetes has stimulated research in the fields of carbohydrate metabolism and the role of insulin in metabolism. Phytochemical screening, antihyperglycemic and antihyperlipidemic effects of aqueous extracts of l. Introduction diabetes mellitus is a group of metabolic disorders characterized by hyperglycemia and defective metabolism of glucose and lipids muhammed and hauwa, 20. Effects of three different doses of shilajit 50, 100 and. Diabetes was induced by a single intraperitoneal injection of alloxan 150 mgkg b.
In 2008, scientists closely examined how alloxan caused diabetes in animals. Optimization of alloxan dose is essential to induce stable diabetes for prolonged period alloxan dose for experimental induction of diabetes in rats has to be optimized. Diabetes was inducing by administering 10,14 freshly prepared alloxan monohydrate 2. Hypoglycemic and hypolipidemic activities of hydroalcoholic. They also improved conditions of dm as indicated by parameters like bodyweight, and lipid profiles along with serum fig 2.
This study was undertaken to investigation the effect of diasulin, a poly herbal drug composed of ethanolic extract of ten medicinal plants on blood glucose, plasma insulin, tissue lipid profile, and lipidperoxidation in alloxan induced diabetes. The effect of orally administered aqueous extracts 400 mgkg body weight of clitoria ternatea leaves and flowers on serum glucose, glycosylated hemoglobin, and insulin were. Antihyperglycemic and antidyslipidemic activity of musa. Research article alloxan induced diabetes mellitus in. The effects of alloxan on the histology of the pancreas. Alloxaninduced diabetes mellitus how is alloxaninduced. Histopathological assessment of the kidney of alloxan induced diabetic rat treated with macerated allium sativum garlic. Antioxidant and hypoglycemic activity of strawberry fruit extracts against alloxan induced diabetes in rats sheriff sheik abdulazeez1 and ponmurugan ponnusamy2 1college of applied medical sciences, shaqra, shaqra university, kingdom of saudi arabia 2ks rangasamy college of technology, thiruchengode, tamil nadu, india.
741 828 28 696 655 1024 1386 880 717 1229 89 563 1324 1290 73 1279 938 445 660 598 1009 221 382 34 1376 276 1008 279 1504 1312 1267 134 370 71 935 178 920 682 30 142 526 476 427 844 845 594 878